Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Deciphering the rules governing assembly order of mammalian septin complexes
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
Department of Cell and Molecular Biology, Karolinska Institute, S-171 77 Stockholm, Sweden.
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
2011 (Engelska)Ingår i: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 22, nr 17, s. 3152-3164Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Septins are conserved GTP-binding proteins that assemble into lateral diffusion barriers and molecular scaffolds. Vertebrate genomes contain 9-17 septin genes that encode both ubiquitous and tissue-specific septins. Expressed septins may assemble in various combinations through both heterotypic and homotypic G-domain interactions. However, little is known regarding assembly states of mammalian septins and mechanisms directing ordered assembly of individual septins into heteromeric units, which is the focus of this study. Our analysis of the septin system in cells lacking or overexpressing selected septins reveals inter-dependencies coinciding with previously described homology subgroups. Hydrodynamic and single-particle data show that individual septins exist solely in the context of stable six-to eight-subunit core heteromers, all of which contain SEPT2 and SEPT6 subgroup members and SEPT7, while heteromers comprising more than six subunits also contain SEPT9. The combined data suggest a generic model for how the temporal order of septin assembly is homology subgroup-directed, which in turn determines the subunit arrangement of native heteromers. Because mammalian cells normally express multiple members and/or isoforms of some septin subgroups, our data also suggest that only a minor fraction of native heteromers are arranged as perfect palindromes.

Ort, förlag, år, upplaga, sidor
American Society for Cell Biology , 2011. Vol. 22, nr 17, s. 3152-3164
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-47388DOI: 10.1091/mbc.E11-03-0253ISI: 000294419300015PubMedID: 21737677Scopus ID: 2-s2.0-80052245849OAI: oai:DiVA.org:umu-47388DiVA, id: diva2:443552
Forskningsfinansiär
VetenskapsrådetTillgänglig från: 2011-09-26 Skapad: 2011-09-20 Senast uppdaterad: 2023-03-23Bibliografiskt granskad
Ingår i avhandling
1. Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
Öppna denna publikation i ny flik eller fönster >>Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
2011 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The cell represents the basic unit of structure and function for all life. The interior of a eukaryotic cell is organized by an extensive array of protein filaments – collectively referred to as the cytoskeleton. These filaments serve diverse essential functions, e.g. to provide mechanical resilience, facilitate intracellular transport, and enable cell polarization, locomotion and division. Here I have explored the mechanisms that regulate synthesis and assembly of two cytoskeletal filament systems – microtubules and septins – and how these interact in human cells. The present thesis is based on three principal discoveries. Firstly, we have found that the microtubule-destabilizing protein Op18/Stathmin also regulates synthesis of tubulin heterodimers, which are the building blocks for microtubules. Secondly, we have unraveled the general rules that govern assembly of mammalian septins into native polymerization-competent heterooligomers. Finally, our combined results point to a non-reciprocal interplay whereby interphase microtubules support a disc-like arrangement of septin filaments, which delineate static plasma membrane regions. I here discuss the physiological significance and implications of these findings.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå universitet, Institutionen för molekylärbiologi, 2011. s. 68
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 1450
Nationell ämneskategori
Cell- och molekylärbiologi
Forskningsämne
cellforskning
Identifikatorer
urn:nbn:se:umu:diva-48508 (URN)978-91-7459-301-3 (ISBN)
Disputation
2011-11-11, Major Groove, Institutionen för Molekylärbiologi, Försörjningsvägen, Byggnad 6L, Umeå Universitet, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2011-10-21 Skapad: 2011-10-20 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

Open Access i DiVA

fulltext(4112 kB)145 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 4112 kBChecksumma SHA-512
2e5989ac39c8d70dccfd91d3f906445b30a75378b87256e7fd1fc6b5f60c64942a45dc9846cb4074de9c1a6173856393299310680cfb636d3484d00254adb933
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Sellin, Mikael E.Sandblad, LindaStenmark, SonjaGullberg, Martin

Sök vidare i DiVA

Av författaren/redaktören
Sellin, Mikael E.Sandblad, LindaStenmark, SonjaGullberg, Martin
Av organisationen
Institutionen för molekylärbiologi (Medicinska fakulteten)
I samma tidskrift
Molecular Biology of the Cell
Cell- och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 145 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 497 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf