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Phosphines are ribonucleotide reductase reductants that act via C-terminal cysteines similar to thioredoxins and glutaredoxins
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.ORCID-id: 0000-0003-1708-8259
2014 (Engelska)Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 4, s. 5539-Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Ribonucleotide reductases (RNRs) catalyze the formation of 2'-deoxyribonucleotides. Each polypeptide of the large subunit of eukaryotic RNRs contains two redox-active cysteine pairs, one in the active site and the other at the C-terminus. In each catalytic cycle, the active-site disulfide is reduced by the C-terminal cysteine pair, which in turn is reduced by thioredoxins or glutaredoxins. Dithiols such as DTT are used in RNR studies instead of the thioredoxin or glutaredoxin systems. DTT can directly reduce the disulfide in the active site and does not require the C-terminal cysteines for RNR activity. Here we demonstrate that the phosphines tris(2-carboxyethyl)phosphine (TCEP) and tris(3-hydroxypropyl)phosphine (THP) are efficient non-thiol RNR reductants, but in contrast to the dithiols DTT, bis(2-mercaptoethyl)sulfone (BMS), and (S)-(1,4-dithiobutyl)-2-amine (DTBA) they act specifically via the C-terminal disulfide in a manner similar to thioredoxin and glutaredoxin. The simultaneous use of phosphines and dithiols results in ~3-fold higher activity compared to what is achieved when either type of reductant is used alone. This surprising effect can be explained by the concerted action of dithiols on the active-site cysteines and phosphines on the C-terminal cysteines. As non-thiol and non-protein reductants, phosphines can be used to differentiate between the redox-active cysteine pairs in RNRs.

Ort, förlag, år, upplaga, sidor
Macmillan Publishers Ltd., 2014. Vol. 4, s. 5539-
Nationell ämneskategori
Annan medicinsk grundvetenskap
Identifikatorer
URN: urn:nbn:se:umu:diva-91043DOI: 10.1038/srep05539ISI: 000338421000012PubMedID: 24986213Scopus ID: 2-s2.0-84903774371OAI: oai:DiVA.org:umu-91043DiVA, id: diva2:733327
Tillgänglig från: 2014-07-09 Skapad: 2014-07-09 Senast uppdaterad: 2023-03-24Bibliografiskt granskad

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Domkin, VladimirChabes, Andrei

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Institutionen för medicinsk kemi och biofysikMolekylär Infektionsmedicin, Sverige (MIMS)
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