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VP3 is crucial for the stability of Nora virus virions
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
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2016 (Engelska)Ingår i: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 223, s. 20-27Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Nora virus is an enteric virus that causes persistent, non-pathological infection in Drosophila melanogaster. It replicates in the fly gut and is transmitted via the fecal-oral route. Nora virus has a single-stranded positive-sense RNA genome, which is translated in four open reading frames. Reading frame three encodes the VP3 protein, the structure and function of which we have investigated in this work. We have shown that VP3 is a trimer that has an α-helical secondary structure, with a functionally important coiled-coil domain. In order to identify the role of VP3 in the Nora virus life cycle, we constructed VP3-mutants using the cDNA clone of the virus. Our results show that VP3 does not have a role in the actual assembly of the virus particles, but virions that lack VP3 or harbor VP3 with a disrupted coiled coil domain are incapable of transmission via the fecal-oral route. Removing the region downstream of the putative coiled coil appears to have an effect on the fitness of the virus but does not hamper its replication or transmission. We also found that the VP3 protein and particularly the coiled coil domain are crucial for the stability of Nora virus virions when exposed to heat or proteases. Hence, we propose that VP3 is imperative to Nora virus virions as it confers stability to the viral capsid.

Ort, förlag, år, upplaga, sidor
Elsevier, 2016. Vol. 223, s. 20-27
Nyckelord [en]
RNA viruses, Nora virus, Capsid stability, Virus biology
Nationell ämneskategori
Immunologi inom det medicinska området
Identifikatorer
URN: urn:nbn:se:umu:diva-124102DOI: 10.1016/j.virusres.2016.06.011ISI: 000383826600003PubMedID: 27329665Scopus ID: 2-s2.0-84977667164OAI: oai:DiVA.org:umu-124102DiVA, id: diva2:949152
Tillgänglig från: 2016-07-17 Skapad: 2016-07-17 Senast uppdaterad: 2023-03-23Bibliografiskt granskad
Ingår i avhandling
1. Biology of a small RNA virus that infects Drosophila melanogaster
Öppna denna publikation i ny flik eller fönster >>Biology of a small RNA virus that infects Drosophila melanogaster
2016 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Drosophila melanogaster has been extensively used as a model organism to study diverse facets of biology, including host-pathogen interactions and the basic biology of its pathogens. I have used the fruit fly as a model to study elementary aspects of Nora virus biology, such as the role of the different proteins encoded by the virus genome. Nora virus, an enteric virus transmitted via the feca-oral route, does not cause any obvious pathology in the fly, although the infection is persistent. Nora virus genome consists of a positive strand RNA that is translated in four open reading frames (ORF).  Since sequence homology studies did not yield much information about the different Nora virus proteins, I have used the cDNA clone of the virus to construct mutants to identify the specific function of each protein. My results have shown that,

1) The protein(s) encoded by ORF 1 are crucial for the replication of the virus genome.

2) The C-terminus of the ORF 1-encoded protein (VP1), is an inhibitor to the RNAi pathway.

3) The transmembrane domain in the N-terminus of the ORF2-encoded protein (VP2) is important for the formation of Nora virus virions.

4) The ORF 3-encoded protein (VP3) forms α-helical trimers and this protein is essential for the stability of Nora virus capsid.                                                    

I have also performed RNA sequencing to investigate the transcriptional response of D. melanogaster in response to Nora virus infection and my results indicate that,                       

5) The upregulation of genes related to cellular stress and protein synthesis and the downregulation of basal digestive machinery, together with the induction of upd3, implies major gut epithelium damage and subsequent regeneration.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2016. s. 52
Nyckelord
Nora virus, Drosophila melanogaster, virus biology, host-pathogen interaction
Nationell ämneskategori
Cell- och molekylärbiologi
Forskningsämne
molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-127352 (URN)978-91-7601-593-3 (ISBN)
Disputation
2016-12-01, Hörsal 135, Byggnad 9A, Norrlands Universitetssjukhus, Umeå, 14:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2016-11-10 Skapad: 2016-11-09 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Sadanandan, Sajna AnandEkström, Jens-OlaJonna, Venkateswara RaoHofer, AndersHultmark, Dan

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Sadanandan, Sajna AnandEkström, Jens-OlaJonna, Venkateswara RaoHofer, AndersHultmark, Dan
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Institutionen för molekylärbiologi (Medicinska fakulteten)Institutionen för medicinsk kemi och biofysik
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