Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A combined experimental atomic force microscopy-based nanoindentation and computational modeling approach to unravel the key contributors to the time-dependent mechanical behavior of single cells
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.ORCID-id: 0000-0001-7719-2600
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.ORCID-id: 0000-0002-9684-6902
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB). (Chondrogenic and Osteogenic Differentiation Group)ORCID-id: 0000-0002-1710-7715
Visa övriga samt affilieringar
2017 (Engelska)Ingår i: Biomechanics and Modeling in Mechanobiology, ISSN 1617-7959, E-ISSN 1617-7940, Vol. 16, nr 1, s. 297-311, artikel-id 27554263Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Cellular responses to mechanical stimuli are influenced by the mechanical properties of cells and the surrounding tissue matrix. Cells exhibit viscoelastic behavior in response to an applied stress. This has been attributed to fluid flow-dependent and flow-independent mechanisms. However, the particular mechanism that controls the local time-dependent behavior of cells is unknown. Here, a combined approach of experimental AFM nanoindentation with computational modeling is proposed, taking into account complex material behavior. Three constitutive models (porohyperelastic, viscohyperelastic, poroviscohyperelastic) in tandem with optimization algorithms were employed to capture the experimental stress relaxation data of chondrocytes at 5 % strain. The poroviscohyperelastic models with and without fluid flow allowed through the cell membrane provided excellent description of the experimental time-dependent cell responses (normalized mean squared error (NMSE) of 0.003 between the model and experiments). The viscohyperelastic model without fluid could not follow the entire experimental data that well (NMSE = 0.005), while the porohyperelastic model could not capture it at all (NMSE = 0.383). We also show by parametric analysis that the fluid flow has a small, but essential effect on the loading phase and short-term cell relaxation response, while the solid viscoelasticity controls the longer-term responses. We suggest that the local time-dependent cell mechanical response is determined by the combined effects of intrinsic viscoelasticity of the cytoskeleton and fluid flow redistribution in the cells, although the contribution of fluid flow is smaller when using a nanosized probe and moderate indentation rate. The present approach provides new insights into viscoelastic responses of chondrocytes, important for further understanding cell mechanobiological mechanisms in health and disease.

Ort, förlag, år, upplaga, sidor
Springer Berlin/Heidelberg, 2017. Vol. 16, nr 1, s. 297-311, artikel-id 27554263
Nyckelord [en]
Cell mechanics, Chondrocyte, Stress relaxation, Atomic force microscopy, Nanoindentation, Poroviscohyperelastic, Finite element analysis
Nationell ämneskategori
Cell- och molekylärbiologi Cellbiologi Biofysik Annan fysik
Forskningsämne
cellforskning; biomekanik
Identifikatorer
URN: urn:nbn:se:umu:diva-125208DOI: 10.1007/s10237-016-0817-yISI: 000394153400021PubMedID: 27554263Scopus ID: 2-s2.0-84983456173OAI: oai:DiVA.org:umu-125208DiVA, id: diva2:967332
Tillgänglig från: 2016-09-08 Skapad: 2016-09-08 Senast uppdaterad: 2023-03-23Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Florea, CristinaTanska, PetriQu, ChengjuanLammi, MikkoKorhonen, Rami

Sök vidare i DiVA

Av författaren/redaktören
Florea, CristinaTanska, PetriQu, ChengjuanLammi, MikkoKorhonen, Rami
Av organisationen
Institutionen för integrativ medicinsk biologi (IMB)
I samma tidskrift
Biomechanics and Modeling in Mechanobiology
Cell- och molekylärbiologiCellbiologiBiofysikAnnan fysik

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 673 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf