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  • 1. Ali, Muhammad Amjad
    et al.
    Azeem, Farrukh
    Nawaz, Muhammad Amjad
    Acet, Tuba
    Abbas, Amjad
    Imran, Qari Muhammad
    Laboratory of Plant Functional Genomics, College of Agriculture & Life Sciences, Kyngpook National University, Buk-gu Daegu, South Korea.
    Shah, Kausar Hussain
    Rehman, Hafiz Mamoon
    Chung, Gyuhwa
    Yang, Seung Hwan
    Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis2018Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 226, s. 12-21Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time PCR, and promoter:GUS lines to analyze expression. Both genes were upregulated in response to abiotic stress. Next, we applied the same stressors to seedlings of T-DNA insertion wrky11 and 17 knock-out mutants (single and double). Under stress, the mutants exhibited slower germination and compromised root growth compared with the wild type. In most cases, double-mutant seedlings were more affected than single mutants. These results suggest that wrky11 and wrky17 are not strictly limited to plant defense responses but are also involved in conferring stress tolerance.

  • 2.
    Chardon, Fabien
    et al.
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    De Marco, Federica
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Marmagne, Anne
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Le Hir, Rozenn
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Vilaine, Françoise
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Bellini, Catherine
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Dinant, Sylvie
    Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
    Natural variation in the long-distance transport of nutrients and photoassimilates in response to N availability2022Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 273, artikel-id 153707Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Phloem and xylem tissues are necessary for the allocation of nutrients and photoassimilates. However, how the long-distance transport of carbon (C) and nitrogen (N) is coordinated with the central metabolism is largely unknown. To better understand how the genetic and environmental factors influence C and N transport, we analysed the metabolite profiles of phloem exudates and xylem saps of five Arabidopsis thaliana accessions grown in low or non-limiting N supply. We observed that xylem saps were composed of 46 or 56% carbohydrates, 27 or 45% amino acids, and 5 or 13% organic acids in low or non-limiting N supply, respectively. In contrast, phloem exudates were composed of 76 or 86% carbohydrates, 7 or 18% amino acids, and 5 or 6% organic acids. Variation in N supply impacted amino acid, organic acid and sugar contents. When comparing low N and non-limiting N, the most striking differences were variations of glutamine, aspartate, and succinate abundance in the xylem saps and citrate and fumarate abundance in phloem exudates. In addition, we observed a substantial variation of metabolite content between genotypes, particularly under high N. The content of several organic acids, such as malate, citrate, fumarate, and succinate was affected by the genotype alone or by the interaction between genotype and N supply. This study confirmed that the response of the transport of nutrients in the phloem and the xylem to N availability is associated with the regulation of the central metabolism and could be an adaptive trait.

  • 3. Igamberdiev, A U
    et al.
    Romanowska, E
    Gardeström, Per
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
    Photorespiratory flux and mitochondrial contribution to energy and redox balance of barley leaf protoplasts in the light and during light-dark transitions2001Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 158, nr 10, s. 1325-1332Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The contribution of mitochondrial oxidation of photorespiratory and respiratory substrates to subcellular energy and redox balance was investigated in leaf protoplasts of barley (Hordeum vulgare L.). The ATP/ADP ratios (indicating the energy balance) in chloroplasts and in tine extrachloroplast compartment were highest in the light in limiting CO2 (photorespiratory conditions), and they drastically increased after illumination if plants were pre-incubated in darkness for 24 hours. After illumination, the ATP/ADP ratio rapidly decreased in chloroplasts. The NADPH/NADP ratio (as an indicator of redox balance) in chloroplasts declined rapidly during the first seconds of darkness, then slowly increased. In limiting CO2, the ratio decreased more slowly during the first minute of darkness corresponding to post-illumination respiratory burst (PIE). During this period, the activation state of chloroplast NADP-malate dehydrogenase was higher in limiting CO2 than in saturating CO2. However, during the light-enhanced dark respiration (LEDR) period, following PIE, there were no differences in subcellular NADPH/NADP ratios in saturating and limiting CO2. A decline in malate and citrate concentrations in protoplasts and activation of mitochondrial NAD-malic enzyme were revealed during LEDR. The results presented highlight the importance of glycine oxidation in mitochondria in energization of the cytosol and chloroplasts and in maintaining redox balance in the light and during the first minute after illumination. And further, they show non-photorespiratory origin of LEDR.

  • 4.
    Kleczkowski, Leszek A.
    et al.
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
    Igamberdiev, Abir U.
    Department of Biology, Memorial University of Newfoundland, NL, St. John's, Canada.
    Magnesium and cell energetics: at the junction of metabolism of adenylate and non-adenylate nucleotides2023Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 280, artikel-id 153901Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Free magnesium (Mg2+) represents a powerful signal arising from interconversions of adenylates (ATP, ADP and AMP). This is a consequence of the involvement of adenylate kinase (AK) which equilibrates adenylates and uses defined species of Mg-complexed and Mg-free adenylates in both directions of its reaction. However, cells contain also other reversible Mg2+-dependent enzymes that equilibrate non-adenylate nucleotides (uridylates, cytidylates and guanylates), i.e. nucleoside monophosphate kinases (NMPKs) and nucleoside diphosphate kinase (NDPK). Here, we propose that AK activity is tightly coupled to activities of NMPK and NDPK, linking adenylate equilibrium to equilibria of other nucleotides, and with [Mg2+] controlling the ratios of Mg-chelated and Mg-free nucleotides. This coupling establishes main hubs for adenylate-driven equilibration of non-adenylate nucleotides, with [Mg2+] acting as signal arising from all nucleotides rather than adenylates only. Further consequences involve an overall adenylate control of UTP-, GTP- and CTP-dependent pathways and the availability of substrates for RNA and DNA synthesis.

  • 5. Krömer, Silke
    et al.
    Lernmark, U
    Gardeström, Per
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
    In-vivo mitochondrial pyruvate-dehydrogenase activity, studied by rapid fractionation of barley leaf protoplasts1994Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 144, nr 4-5, s. 485-490Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Inactivation and activation of mitochondrial and chloroplastic pyruvate dehydrogenase (PDH; EC 1.2.4.1) have been studied in isolated organelles and protoplasts from barley leaves. The pyruvate dehydrogenase complex (PDC) from barley leaf mitochondria was inactivated by ATP (65% at 4 mM ATP) while the chloroplastic PDC was stimulated (75% at 4 mM ATP), MgCl2 inhibited the mitochondrial complex with increasing concentrations while the chloroplastic complex was stimulated. MnCl2 had a stimulatory effect on both PDCs. ATP-inactivated mitochondrial PDC could be reactivated by MnCl2, but not by MgCl2 or CaCl2. The major part of the PDC was located in the chloroplast. The in vivo mitochondrial PDC activity could be determined after removal of the chloroplastic isoform by subcellular fractionation. This activity was sensitive to ATP inhibition confirming the mitochondrial origin. The in vivo PDC activity as well as the ATP sensitivity did not change when protoplasts were incubated in darkness or illuminated in photorespiratory or non-photorespiratory conditions. This was taken as an indication of an unchanged activation state of the enzyme under the conditions tested.

  • 6.
    Oquist, Gunnar
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
    EFFECTS OF WINTER STRESS ON CHLOROPHYLL ORGANIZATION AND FUNCTION IN SCOTS PINE1986Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 122, nr 2, s. 169-179Artikel i tidskrift (Refereegranskat)
  • 7.
    Shaikhali, Jehad
    et al.
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik.
    Baier, Margarete
    Ascorbate regulation of 2-Cys peroxiredoxin-A promoter activity is light-dependent2010Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 167, nr 6, s. 461-467Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The 2-Cys peroxiredoxin-A (2CPA) promoter is a model promoter to study redox and ABA-dependent stress signaling. Here, an Arabidopsis reporter gene line expressing luciferase under control of the 2CPA promoter was used to study the impact of ascorbate on reporter gene transcription in a series of protoplast and leaf slice incubation experiments. It was shown that ascorbate has a dual function on gene expression regulation. First, a comparison of responses to ascorbate, dehydroascorbate and reduced and oxidized glutathione demonstrated that ascorbate feeding supports gene expression regulation by increasing the catalytic capacity in redox signaling, as defined by the concentration of low molecular weight antioxidants and their oxidized counterparts. Second, ascorbate had a specific and light-dependent effect on 2CPA transcription, which cannot be substituted by reduced glutathione. Based on the differences between ascorbate and glutathione in the subcellular redox-cycling capacities, it is concluded that ascorbate feeding modulates chloroplast-specific regulation of 2CPA expression. (C) 2009 Elsevier GmbH. All rights reserved.

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